Coding

Part:BBa_K3733000:Design

Designed by: Zhenhao Han   Group: iGEM21_HZAU-China   (2021-09-27)


NarX: The membrane-associated sensors in the presence of nitrate


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We optimized the codon of NarX based on Escherichia coli codon bias.


Source

The DNA sequence gets from MG1655 genome.

References

Cheung J, Hendrickson W A. Structural analysis of ligand stimulation of the histidine kinase NarX[J]. Structure, 2009, 17(2): 190-201.